Journal: bioRxiv

Article Title: Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell Receptors

doi: 10.64898/2026.01.29.702605

Figure Lengend Snippet: Analysis of HIV-specific TCR avidity and functional affinity . ( A ) Estimated TCR-pHLA binding strength for individual clonotypes, calculated as the average ratio of bound tetramer (antigen, Ag) UMIs to CD3 UMIs. Statistical comparisons were performed using a non-parametric t-test. Each donor contribution is depicted with a different colour matched to the donor. ( B ). Representative FACs plot showing a monoclonal Jurkat TCR stained with a non-specific control tetramer (B*42:01-TL9) and the cognate tetramer (B*58:01-KW11). TCR-engineered T cells were co-cultured with HLA-B*58:01 monoallelic antigen-presenting cells pulsed with graded peptide concentrations (2μM to 0.125μM) at a 1:1 ratio for 18 hours. The histograms illustrate dose-dependent CD69 expression for 726_KW11-specific TCR3 clone. ( C and D ). Representative peptide dose-response curves for ( C ) IW9-specific TCRs and ( D ) KW11-specific TCRs derived from early-treated donor 726 and late-treated donor 309. TCR identifiers correspond to clonotypes with paired α and β-chains confirmed by both Illumina and Oxford nanopore sequencing. ( E and F ) Representative steady-state SPR measurements showing binding interactions between soluble HLA-B*58:01-IW9 pHLA complex and four IW9-specific TCRs, two from the early ART-treated donor 726 ( E ) and two from the late ART-treated donor 309 ( F ). Individual plots indicate donor TCR IDs and corresponding dissociation constants (K D )

Article Snippet: The concentrated lentivirus, along with 4 μg/mL of polybrene (Sigma-Aldrich, St Louis, MO), was then used to transduce a TCR-null Jurkat cell line (BPS Bioscience Cat. No: 78539).

Techniques: Functional Assay, Binding Assay, Staining, Control, Cell Culture, Expressing, Derivative Assay, Nanopore Sequencing

Journal: bioRxiv

Article Title: Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell Receptors

doi: 10.64898/2026.01.29.702605

Figure Lengend Snippet: Normalized dose-response curves showing CD69 upregulation in monoclonal TCR-expressing Jurkat cells stimulated with IW9 (top) or KW11 (bottom) peptides. The Y-axis indicates the % of CD69 + Jurkat cells, and the X-axis represents peptide concentration. Each curve corresponds to an individual TCR, labeled by its respective ID. Red curves denote TCRs derived from early-treated donors, while blue curves represent TCRs from late-ART-treated donors. Peptide dose-response curves for each TCR were generated to estimate antigen sensitivity, with EC 50 serving as a quantitative proxy for TCR functional responsiveness.

Article Snippet: The concentrated lentivirus, along with 4 μg/mL of polybrene (Sigma-Aldrich, St Louis, MO), was then used to transduce a TCR-null Jurkat cell line (BPS Bioscience Cat. No: 78539).

Techniques: Expressing, Concentration Assay, Labeling, Derivative Assay, Generated, Functional Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma

doi: 10.1007/s00262-021-03054-8

Figure Lengend Snippet: DARA-mediated ADCC based on CD16 expressing NK cells. A BCBL-1, GTO, and TY-1 cells were incubated with various concentrations of DARA, and co-cultured with ADCC-indicating Jurkat cells (E:T ratio = 1:5) for 18 h. Luciferase activity was measured. B, C, D, E) CFSE-labeled BCBL-1 cells were treated with IgG1 or DARA, co-cultured with CD16 + NK (N6) or CD16- NK (KHYG-1) cell lines at different E:T ratio for 4 h, and analyzed for dead BCBL-1 (CFSE+Ghost Dye+) cells by flow cytometry. F Up-regulation of CD107a after DARA treatment was analyzed by flow cytometry. G Percentages of CD56+CD107a+ cells after stimulation with DARA (n = 3). Data are presented as mean values ± standard error (SE). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 significant levels

Article Snippet: Recombinant Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay.

Techniques: Expressing, Incubation, Cell Culture, Luciferase, Activity Assay, Labeling, Flow Cytometry